Coding

Part:BBa_K2130009:Design

Designed by: Ooi Kean Hean   Group: iGEM16_NTU-Singapore   (2016-10-19)


∆HNH ∆REC2 SP-dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 251
    Illegal BglII site found at 804
    Illegal BamHI site found at 1622
    Illegal XhoI site found at 2770
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1966
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2112
    Illegal BsaI site found at 2966
    Illegal BsaI.rc site found at 1027


Design Notes

Assmebled via Gibson


Source

Addgene

References