Coding
Part:BBa_K2130009:Design
Designed by: Ooi Kean Hean Group: iGEM16_NTU-Singapore (2016-10-19)
∆HNH ∆REC2 SP-dCas9
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 251
Illegal BglII site found at 804
Illegal BamHI site found at 1622
Illegal XhoI site found at 2770 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1966
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2112
Illegal BsaI site found at 2966
Illegal BsaI.rc site found at 1027
Design Notes
Assmebled via Gibson
Source
Addgene